Bacterial species and resistances

Bacterial Infections

We work on identifying the bacterial species causing an infection including their antibiotic resistances

Bacterial Species & Antibiotic Resistances

Our cartridge amp.flow is equipped with an amplification chamber, a sensor area to attach a slide containing a microarray with capture probes, as well as numerous reservoirs for reagent storage.

Amplification, labeling and hybridization can take place on the cartridge and run with an automated protocol.

Pumping, heating and spot detection are combined within the universal cartridge reader UCAR, minimizing manual steps.

The following examples show the successful detection of PCR products of

A. baumannii with NDM resistance (New Delhi metallo-beta-lactamase), as well as
S. aureus without methicillin resistance (MSSA),
S. aureus with methicillin resistance (MRSA), and
S. hominis with methicillin resistance

using the same microarray layout and assay. The cartridges were controlled and read by our universal cartridge reader UCAR. The microarray was automatically processed to quantify the spot signals followed by determining the species and resistances. For S. aureus, a single nucleotide polymorphism (SNP) detection is included to distinguish S. aureus from other staphylococcus species. S. hominis is also selectively detected by an additional SNP probe set.